| 水稻染色体标本制备的风油精法 |
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| 引用本文： | 庄南生,黎京度.水稻染色体标本制备的风油精法[J].Acta Botanica Sinica,1992(4). |
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| 作者姓名： | 庄南生 黎京度 |
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| 作者单位： | 西南农业大学生物学基础部，西南农业大学生物学基础部 重庆 630716，重庆 630716 |
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| 摘 要： | 水稻的染色体较小,不同的染色体在形态上较难区分。常规的压片技术由于很难使染色体分散,且也不能完全排除细胞质的干扰,因而很不适用于水稻染色体核型分析及显带。Kurata 等(1978)采用酶解与火焰干燥技术制备水稻染色体标本,获得清晰的染色体图象,从而成功地进行了水稻染色体的核型分析。陈瑞阳等(1982)参照人类染色体
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| 关 键 词： | 水稻 染色体标本 风油精法 |
AN ESSENTIAL BALM METHOD FOR PREPARING RICE CHROMOSOMES |
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| Authors: | Zhuang Nan-sheng Li Jing-du |
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| Abstract: | An essential balm(EB)method was described for preparing mitotic chromosomes in rice (Oryza sativa L.).Briefly,root tips were pretreated with EB,fixed in 3:1 methanol-acetic acid. Chromosome specimens were made by squash and smear technique without cell wall degrada- tion,and then stained with Giemsa.The G-bands of chromosomes could be distinctly visible with this method. |
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| Keywords: | Rice Chromosome preparation Essential balm method |
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