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Adipose tissue-derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: An optimization and comparison method
Authors:Ahad Ferdowsi Khosroshahi  Jafar Soleimani Rad  Razie Kheirjou  Babak Roshangar  Morteza Rashtbar  Roya Salehi  Mohammad Reza Ranjkesh  Leila Roshangar
Affiliation:1. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;2. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;3. Stem Cell and Regenerative Medicine Research Center, Guilan University of Medical Science, Rasht, Iran;4. Department of Medical Nanotechnology, Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran;5. Department of Dermatology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:
The extracellular matrix of different mammalian tissues is commonly used as scaffolds in the field of tissue engineering. One of these tissues, which has frequently been studied due to its structural and biological features, is the small intestine submucosal membrane. These research are mainly done on the porcine small intestine. However, a report has recently been published about a scaffold produced from the submucosal layer of the ovine small intestine. In the present study, ovine small intestine submucosal (OSIS) was decellularized in a modified manner and its histological, morphological, and biomechanical properties were studied. Decellularization was performed in two phases: physical and chemical. In this method, a chloroform-methanol mixture, enzymatic digestion, and a constant dose of sodium dodecyl sulfate (SDS) was used in the least agitation time and its histological property and biocompatibility were evaluated in the presence of adipose tissue-derived stem cells (ADSCs); furthermore, ADSCs were isolated with a simple method (modified physical washing non-enzymatic isolation). The results were showed that the use of OSIS could be effective and operative. Mechanical properties, histological structure and shape, and glycosaminoglycan content were preserved. In the SDS-treated group, more than 90% of the native cells of tissue were deleted, and also in this group, no toxicity was observed and cell proliferation was supported, compared to the untreated group. Therefore, our results indicate that ADSCs seeded on OSIS scaffold could be used as a new approach in regenerative medicine as hybrid or hydrogel application.
Keywords:acellular small intestine submucosal scaffold  adipose tissue-derived mesenchymal stromal cells  sheep  tissue engineering
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