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Protective effects of irisin on hypoxia-reoxygenation injury in hyperglycemia-treated cardiomyocytes: Role of AMPK pathway and mitochondrial protection |
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| Authors: | Jiamao Fan Qing Zhu Zhenhua Wu Jiao Ding Shuai Qin Hui Liu Pengfei Miao |
| Institution: | 1. Department of Cardiology, Linfen Central Hospital, Linfen, China;2. Department of Cardiology, Linfen Central Hospital, Linfen, China
Institutes of Biomedical Sciences, Shanghai Medical School, Fudan University, Shanghai, China Jiamao Fan and Qing Zhu contributed equally to this study.;3. Department of Cardiovascular Surgery, Linfen Central Hospital, Linfen, China |
| Abstract: | Recent evidence has verified the cardioprotective actions of irisin in different diseases models. However, the beneficial action of irisin on hypoxia-reoxygenation (HR) injury under high glucose stress has not been described. Herein our research investigated the influence of irisin on HR-triggered cardiomyocyte death under high glucose stress. HR model was established in vitro under high glucose treatment. The results illuminated that HR injury augmented apoptotic ratio of cardiomyocyte under high glucose stress; this effect could be abolished by irisin via modulating mitochondrial function. Irisin treatment attenuated cellular redox stress, improved cellular ATP biogenetics, sustained mitochondria potential, and impaired mitochondrion-related cell death. At the molecular levels, irisin treatment activated the 5′-adenosine monophosphate-activated protein kinase (AMPK) pathway and the latter protected cardiomyocyte and mitochondria against HR injury under high glucose stress. Altogether, our results indicated a novel role of irisin in HR-treated cardiomyocyte under high glucose stress. Irisin-activated AMPK pathway and the latter sustained cardiomyocyte viability and mitochondrial function. |
| Keywords: | AMPK pathway cardiomyocyte HR injury irisin mitochondrial function |