Development of a highly specific diagnostic 23S rDNA oligonuleotide probe for Mycobacterium leprae |
| |
Authors: | W Liesack C Pitulle E Stackebrandt |
| |
Institution: | Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universit?t, Kiel, FRG. |
| |
Abstract: | A 21-mer DNA oligonucleotide probe targeting the 23S rRNA of Mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. Even under relaxed hybridization and washing conditions (20 degrees C below Tm) the probe was highly selective in that positive signals were only detected with M. leprae, about half of the slow-growing and one of the fast-growing reference mycobacteria and Gordona bronchialis. At more stringent washing temperatures (6 degrees C below Tm) only the rRNA of Mycobacterium leprae was detectable. |
| |
Keywords: | |
|
|