Effect of tolbutamide on fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase in rat liver |
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| Authors: | K Kaku M Matsuda A Matsutani T Kaneko |
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| Affiliation: | 1. Department of Gastrointestinal and General Surgery, Wroclaw Medical University, ul. Sklodowskiej-Curie 66, 50-369 Wroclaw, Poland;2. Laboratory of Cytobiochemistry, Biotechnology Faculty, University of Wroclaw, Wroclaw, Poland;1. Assistant professor of orthopedic surgery, Department of orthopedic surgery Qom University of Medical Sciences, Qom, Iran;2. Assistant professor of orthopedic surgery, Department of orthopedic surgery Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Digestive Disease Research Institute, Shariati Hospital, Tehran, Iran |
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| Abstract: | The effects of tolbutamide on the activities of fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase were examined using rat hepatocytes. Tolbutamide stimulated fructose-6-phosphate,2-kinase activity and inhibited fructose-2,6-bisphosphatase activity, resulting in an increase of fructose-2,6-bisphosphate level. Changes in the activities of the enzyme by tolbutamide were due to variation in the Km value, but not dependent on alteration of Vmax. Glucagon inhibition of fructose-2,6-bisphosphate formation resulting from an inactivation of fructose-6-phosphate,2-kinase and an activation of fructose-2,6-bisphosphatase was released by tolbutamide. Tolbutamide stimulation of fructose-2,6-bisphosphate formation through regulation of fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase may produce enhancement of glycolysis and inhibition of gluconeogenesis in the liver. |
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