<Emphasis Type="Italic">Acmella radicans</Emphasis> var. <Emphasis Type="Italic">radicans: In vitro</Emphasis> culture establishment and alkamide content |
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Authors: | P?Rios-Chavez E?Ramirez-Chavez C?Armenta-Salinas Email author" target="_blank">J?Molina-TorresEmail author |
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Institution: | (1) Departamento de Biotecnología y Bioquímica, CINVESTAV-IPN, U. Irapuato, Apdo. Postal 629, CP 36500 Irapuato, Gto., Mexico;(2) Instituto de Investigaciones Químico-Biológicas, Universidad de San Nicolás de Hidalgo Edificio B-2, Ciudad Universitaria, 58030 Morelia, Mich., Mexico |
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Abstract: | Summary
Acmella radicans var. radicans propagation was established in vitro. This plant belongs to the Asteraceae from which some species are known for their insecticide, fungicide and antibacterial
activity. The complete Murashige and Skoog (MS) medium was the best in assisting seed germination. In order to obtain shoots
in vitro, a complete MS medium and half-strength MS medium were assayed with explants from leaves, nodes, and internodes. The best
medium for shoot production was the half-strength MS medium with no addition of plant growth regulators, and the highest shoot
propagation was from single-node explants. Regeneration of roots on shoot explants in the medium was obtained without the
addition of growth regulators. Of the plantlets that were acclimated, 90% of them were obtained from rooted shoots with completely
expanded leaves. The alkamide content was evaluated for each tissue and the higher concentration was observed in flower heads.
The main alkamides present in the leaves and the flower heads were N-(2-phenylethyl)-2Z,4E-octadienamide and 3-phenyl-N-(2-phenylethyl)-2-propenamide. This study describes the methodology for the establishment and propagation of Acmella radicans in vitro and the evaluation of different tissue alkamide contents in vitro and in the field. |
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Keywords: | affinin single-node N-isobutyl-2E-decamonoenamide N-isobutyl-decamide bioactive metabolite Asteraceae NMR GC/MS |
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