Purification and characterization of a dimethylsulfoniopropionate cleaving enzyme from Desulfovibrio acrylicus |
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| Authors: | Marc JEC van der Maarel Walter Aukema Theo A Hansen |
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| Institution: | Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas C.P. 6109, Campinas —São Paulo, Brazil; Departamento de Histologia e Embriologia, Universidade Estadual de Campinas C.P. 6109, Campinas —São Paulo, Brazil; Departamento de Patologia Geral, Universidade Estadual de Londrina, 86051, Londrina, PR, Brazil |
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| Abstract: | Abstract Enterotoxigenic Escherichia coli (STa+) strains were isolated from adult bovine with diarrhea. These strains did not express any known ETEC-specific adhesins. Although hemagglutination with rat and sheep erythrocytes was observed in the presence of D-mannose (MRHA), these strains also showed mannose-sensitive hemagglutination (MSHA) with guinea-pig erythrocytes. Electron microscopic studies revealed the presence of fimbria-like structures (provisionally called "F43ms") on bacterial cells grown at 37°C but not on cells grown at 18°C. However, it was observed by SDS-PAGE that the J-1 strain (F43ms+) produces a protein similar to F1 fimbriae, and this strain hybridized with a DNA probe for F1 fimbriae. Immunogold-labelling techniques indicated that a rabbit anti-serum is specific for F43ms fimbrial structures, but not for Type 1 fimbriae. The immunofluorescence test carried out with semipurified F43ms on bovine brush borders suggests that the fimbria-like structures are responsible for the adhesion to bovine epithelial cells. |
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| Keywords: | Fimbriae ETEC Escherichia coli MSHA |
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