局灶性脑缺血再灌损伤时神经细胞内Ca~(2+)时空动态变化的实验研究

本文用插线法制作局灶性脑缺血／再灌损伤模型，利用激光共聚焦扫描显微镜观察活体脑片细胞内Ｃａ２＋的分布及动态变化，结果表明：（１）缺血／再灌时间不同，梗塞面积不同，缺血４小时梗塞面积占同侧半球的１６．３％，缺血４小时再灌２０小时梗塞面积增加到２５．９％，缺血２４小时梗塞面积占同侧半球的６０．４％。（２）本文首次观察到在缺血４小时纹状体区域的Ｃａ２＋变化明显高于皮层，并且再灌后皮层及纹状体区域Ｃａ２＋的含量明显增加

THE EXPERIMENTAL STUDY ON SPATIOTEMPORAL CHANGES OF INTRACELLULAR Ca 2+ IN NEURONS DURING FOCAL ISCHEMIA/REPERFUSION DAMAGE

The model of rat focal cerebral ischemia/reperfusion damage was established by reversible inserting of a nylon thread, and the distribution and dynamic changes of intracellular Ca 2+ in brain slice of focal ischemia were observed using laser confocal imaging system. The main results were: (1) Infarct area increases with the extension of time of ischemia reperfusion. At 4 hours after MCAO, the infarct area is 16 3% of ipsilateral hemisphere, after 20 hours of reperfusion, the area increases to 25 9%. At 24 hours after MCAO, the infarct area is 60 4% of ipsilateral hemisphere. The abnormal infarct region is demonstrated only in striatum but not in cortex at 4 hours after the occlusion; at 24 hours, the abnormal region is shown in both areas. (2) For the first time we discover that intracellular Ca 2+ is more concentrated in striatum than in cortex at 4 hours. After 4 hours ischemia is followed by 20 hours reperfusion, the increases of intracellular Ca 2+ in both regions are more distinct than those at 4 hours.