Glutamate dehydrogenase from yeast and from animal tissues |
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| Affiliation: | 1. Bioengineering and Environmental Science Laboratory, CEEFF, CSIR- Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India;1. Laboratory of Genetics and Biotechnology, EMBRAPA Agroenergy, Brasília, Brazil;2. Graduate Program of Chemical and Biological Technologies, Institute of Chemistry, University of Brasília, Brazil;3. Graduate Program of Microbial Biology, Department of Cell Biology, Institute of Biology, University of Brasília, Brazil |
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| Abstract: | The purification of glutamate dehydrogenase from yeast, beef intestinal mucosa and from beef brain is presented. A comparative study of stability of glutamate dehydrogenase from the indicated sources and from beef liver is presented. In all cases reduced NADP markedly decreases enzyme stability. The possible significance of these findings in relation to protein turnover are briefly discussed. Some of the kinetic and molecular parameters of yeast glutamate dehydrogenase are presented. |
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