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Accelerated liquid isolectric focusing using a direct current source that maintain constant power. |
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| Authors: | A D Strosberg R A Jeffery L J Freier W J Connolly |
| Affiliation: | 1. Cardiac Unit, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;2. the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114 USA;3. Skipper B Group, C. S. Draper Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA |
| Abstract: | A power supply has been designed to improve liquid isoelectric focusing by automatically maintaining constant power applied to the column. The main advantages are (a) a reduced exposure time which is particularly important when sensitive proteins are being resolved; (b) a reduction in the loss of resolution due to diffusion or denaturation of the proteins; and (c) a considerable increase in efficiency of the use of the equipment for preparative purposes. |
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