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An integrated SSR based linkage map for <Emphasis Type="Italic">Zoysia matrella</Emphasis> L. and <Emphasis Type="Italic">Z. japonica</Emphasis> Steud.
Authors:Manli Li  Nana Yuyama  Mariko Hirata  Yunwen Wang  Jianguo Han  Hongwei Cai
Institution:(1) Department of Grassland Science, College of Animal Science and Technology, China Agricultural University, 2 Yuanmingyuan West Road, 100193 Beijing, China;(2) Forage Crop Research Institute, Japan Grassland Farming and Forage Seed Association, 388-5, Higashiakata, Nishinasuno, Tochigi 329-2742, Japan;(3) Department of Plant Genetics and Breeding, College of Agriculture and Biotechnology, China Agricultural University, 2 Yuanmingyuan West Road, 100193 Beijing, China;
Abstract:Japanese lawngrass (Zoysia japonica) and Manila grass (Z. matrella) are the two most important and commonly used Zoysia species. A consensus based SSR linkage map was developed for the genus by combining maps from each species. This used previously constructed maps for two Z. japonica populations and a new map from Z. matrella. The new SSR linkage map for Z. matrella was based on 86 F2 individuals and contained 213 loci and covered a map distance of 1,351.2 cM in 32 linkage groups. Comparison of the three linkage maps constructed from populations with different genetic backgrounds indicated that most markers exhibited a consensus order, although some intervals or regions displayed discrepancy in marker orders or positions. The integrated map comprises 507 loci with a mean interval of 4.1 cM, covering a map distance of 2,066.6 cM in 22 linkage groups. The SSR-based map will allow marker-assisted selection and be useful for the mapping and cloning of economically important genes or quantitative trait loci.
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