HUMAN SPERM β-GLUCURONIDASE IS POORLY EXTRACTABLE BY TRITON X-100 |
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Authors: | ADRIANO BRANDELLI PATRICIA V MIRANDA JORGE G TEZ
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Institution: | ADRIANO BRANDELLI,PATRICIA V. MIRANDA,JORGE G. TEZÓN |
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Abstract: | A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1m KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3m KCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction. |
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Keywords: | glycosidase enzyme sperm detergent cytoskeleton |
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