Solid-phase Synthesis and Cellular Localization of a C- and/or N-terminal Labelled Peptide |
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Authors: | P Vidal L Chaloin J Mry N Lamb N Lautredou R Bennes F Heitz |
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Institution: | P. Vidal,L. Chaloin,J. Méry,N. Lamb,N. Lautredou,R. Bennes,F. Heitz |
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Abstract: | We report the solid-phase synthesis by the Fmoc strategy of a peptide containing a cysteamide group at its C-terminus. This peptide was subject to further modifications including the linkage of fluorophores, namely lucifer yellow and coumarin respectively, at the C- and/or N-terminals. After incubation with living cultured cells these two probes were localized and it is concluded that the post-synthesis modifications can strongly modify the localization of the peptide. |
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Keywords: | SPPS labelled peptides cellular localization amphipathic peptide |
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