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Hydroxylations of substituted naphthalenes by Escherichia coli expressing aromatic dihydroxylating dioxygenase genes from polycyclic aromatic hydrocarbon-utilizing marine bacteria |
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| Authors: | Kazutoshi Shindo Ayako Osawa Yuki Kasai Nobuko Iba Ayako Saotome Norihiko Misawa |
| Affiliation: | aDepartment of Food and Nutrition, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan bMarine Biotechnology Institute, 3-75-1, Heita, Kamaishi 026-0001, Japan |
| Abstract: | Bioconversion experiments of various mono- or di-substituted naphthalenes such as dimethylnaphthalenes were carried out using the cells of Escherichia coli that expressed aromatic dihydroxylating dioxygenase genes (phnA1A2A3A4 and phdABCD) from polycyclic aromatic hydrocarbon-utilizing marine bacteria, Nocardioides sp. KP7 and Cycloclasticus sp. A5, respectively. We found that the former dioxygenase PhnA1A2A3A4 had broad substrate preference for these compounds and often was able to hydroxylate their methyl groups. Specifically, 1,4-dimethylnaphthalene was predominantly bioconverted into 1,4-dihydroxymethylnaphthalene. |
| Keywords: | Polycyclic aromatic hydrocarbon Aromatic dihydroxylating dioxygenase Dimethylnaphthalene Cycloclasticus Nocardioides |
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