汉坦病毒HTN261株S基因片段的核苷酸序列测定及分析

黑龙江省是肾综合征出血热(HFRS)的重疫区。近年来HFRS年的发病人数曾超过万人。流行病学和血清学研究表明黑龙江省HFRS疫区主要是姬鼠型，但目前尚缺乏病毒的分子生物学资料。我们对从疫区捕获的宿主动物-黑线姬鼠肺中分离的汉坦病毒HTN261株的S基因片段的全基因序列进行了测定和初步分析。结果如下，HTN261株的S基因片段的全序列长为1697nt。只有一个主要的编码N蛋白的ORF，起始位置为第37nt，终止于1326nt，编码的蛋白长为429aa。没有发现存在ORF2。HTN261株的S基因片段核苷酸序列与HTN型中的病毒株的同源性很高，而与汉坦病毒其他型的同源性较差。从种系发生树分析来看，HTN261株归结于汉坦病毒的HTN型。在HTN型之内，HTN261株和HTN76-118株在一个分枝内。就其核苷酸和蛋白的同源性来说，HTN261株和HTN76-118株的同源性分别是89%(全S基因)和98%(蛋白)。而与中国境内发现的其他汉坦病毒株Z10，HU，Chen4，NC167等基因和蛋白的同源性相对较差。汉坦病毒除具有其宿主的依赖性外，还具有其地理的簇集性。HTN261株和HTN76-118株之间S基因和N蛋白序列的变异性的差异分别为11%和2%，表明HTN261株和HTN76-118株还有不同，可能是不同的亚型。不过，尚有待于进一步研究证明。

Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China

To determine the complete nucleotide sequence of the S genome segment of HTN261 virus strain which was isolated from the striped field mouse (Apodemus agraius) in Heilongjiang province of the Northeast China, the complete nucleotide sequence of the S genome segment of HTN261 virus strain was determined with two clones, which were obtained using RT-PCR, and by linking two overlapping fragments into PUC19 vector respectively. The complete nucleotide sequence of the S genome segment of HTN261 virus strain was 1697 nucleotides in length and contained one ORF encoding the N protein starts at position 37 and ends at position 1326. The length of predicted protein, 429 amino acids, is identical to the N protein of HTN, DOB, SEO types of hantaviruses. Comparison of S segment sequence of HTN261 strain with the corresponding sequences of other hantaviruses showed that the identities of HTN261 strain with HTN76-118 strain were 89% (complete nucleotide sequence) andd 98% (amino acid sequence). Phylogenetic analysis showed that HTN261 strain with HTN76-118 strain form one lineage within the clade containing hantaviruses of HTN type. But the identities of HTN261 strain with HTN76-118 strain differ by 11% in complete nucleotide sequence of S segment and 2% in amino acid sequence of N protein. HTN261 strain belongs to hantavirus HTN type and may be a new subtype.