Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli |
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| Authors: | P Montoro N Teinseree W Rattana P Kongsawadworakul N Michaux-Ferriere |
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| Institution: | (1) Rubber Project, CIRAD/KAPI, Kasetsart University, Bangkok 10900, Thailand Fax: +662-9428684 e-mail: pascal.montoro@cirad.fr, TH;(2) Department of Agriculture, Chatuchak, Bangkok 10900, Thailand, TH;(3) Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10900, Thailand, TH;(4) BIOTROP, CIRAD, 34032 Montpellier, France, FR |
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| Abstract: | The influence of CaCl2 was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl2.Five A. tumefaciens strains (C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the
strain EHA105pC2301 was selected to conduct further experiments. The calli were precultured on MM containing a range of CaCl2 concentrations, then inoculated with Agrobacterium suspension. Transfer of friable calli from MM containing 9 mM CaCl2 to calcium-free medium significantly enhanced the transient β-glucuronidase activity. Interestingly, the use of calcium-free Agrobacterium resuspension medium to inoculate friable calli again dramatically increased the transformation efficiency. Induction of Agrobacterium's virulence with acetosyringone remained an important factor to stimulate transformation.
Received: 14 October 1999 / Revision received: 3 January 2000 / Accepted: 4 January 2000 |
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| Keywords: | Rubber tree Agrobacterium tumefaciens calcium gene transfer |
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