PAI-1 inhibits urokinase-induced chemotaxis by internalizing the urokinase receptor |
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Authors: | Degryse B Sier C F Resnati M Conese M Blasi F |
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Institution: | Molecular Genetics Unit, DIBIT, Department of Cell Biology and Functional Genetics, University Vita-Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy. |
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Abstract: | PAI-1 (plasminogen activator inhibitor-1) binds the urokinase-type plasminogen activator (uPA) and causes its degradation via its receptor uPAR and low-density lipoprotein receptor-related protein (LRP). While both uPA and PAI-1 are chemoattractants, we find that a preformed uPA-PAI-1 complex has no chemotactic activity and that PAI-1 inhibits uPA-induced chemotaxis. The inhibitory effect of PAI-1 on uPA-dependent chemotaxis is reversed when uPAR internalization is inhibited by the 39 kDa receptor-associated protein or by anti-LRP antibodies. Under the same conditions, the uPA-PAI-1 complex is turned into a chemoattractant causing cytoskeleton reorganization and extracellular-regulated kinase/mitogen-activated protein kinases activation. Thus, uPAR internalization by PAI-1 regulates cell migration. |
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