bvg-Negative Regulation of Repeated Sequence Transposition in Bordetella pertussis Cells |
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| Authors: | L. N. Sinyashina V. V. Vorontsov E. G. Semin A. V. Chestkov Yu. D. Tsygankov G. I. Karataev |
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| Affiliation: | (1) Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, 123098, Russia;(2) State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia |
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| Abstract: | A method of monitoring the sequential events of IS481 transposition into the ctag site of bvg operon of Bordetella pertussis has been developed. Reproduction of virulent B. pertussis cells in vitro is accompanied by intrachromosomal site-specific IS481 transposition, which, in turn, results in inactivation of bvg operon of the causative agent and cell avirulent state. Avirulent bvg mutants of B. pertussis are incapable of intramolecular IS481 transposition. The frequency of the transposition increases when MgSO4 and nicotinic acid are present the culture medium. In the absence of these modulating factors, IS481 transposition along B. pertussis chromosome is inhibited but not arrested completely. Negative regulation of the bvg-repressed genes of B. pertussis seems to be a mechanism that controls bvg-dependent IS481 transposition. |
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