Variation in blood proteins and mitochondrial DNA within and between local populations of longtail macaques,Macaca fascicularis on the Island of Java, Indonesia |
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Authors: | Dyah Perwitasari-Farajallah Yoshi Kawamoto Bambang Suryobroto |
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Institution: | (1) Present address: Department of Biology, Bogor Agricultural University, Jalan Raya Pajajaran, 16143 Bogon, Indonesia;(2) Primate Research Institute, Kyoto University Inuyama, 484-8506 Aichi, Japan;(3) Present address: Primate Research Institute, Kyoto University, Inuyama, 484-8506 Aichi, Japan |
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Abstract: | We examined 31 blood protein loci, and restriction fragment length profiles of a PCR product of mitochondrial DNA containing
the D-loop region using five kinds of restriction endonucleases (HaeIII,HinfI,MboII,MspI, andSau3 AI) in order to quantify the level of genetic variation of longtail macaques,Macaca fascicularis. Samples were collected from nine social groups in five localities of West Java, Indonesia. The average heterozygosity per
individual
was 0.060 and 15.7% of the loci were polymorphic (Ppoly) over all populations in the protein analysis. There was no mtDNA haplotype variation within either social groups or local
populations. To the contrary, great diversity was observed among local populations. Both nuclear diversity (measured byNei's standard genetic distance) and mitochondrial diversity (measured by sequence divergence) showed a significantly positive
correlation with geographic distance. There was no significant correlation between these two genetic markers, however. The
genetic structure of the population was evaluated in terms of local inbreeding and temporal changes in allele frequency. Differences
between nuclear and mitochondrial data are discussed in relation to gender specific migration and lineage sorting. |
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Keywords: | Longtail macaques Blood proteins mtDNA Genetic variation Java Island |
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