Urease immobilized on nylon: preparation and properties |
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| Authors: | A. Anita C. A. Sastry M. A. Hashim |
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| Affiliation: | Institute of Advanced Studies University of Malaya 50603 Kuala Lumpur,
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| Abstract: | Urease (EC 3.5.1.5) was covalently attached through glutaraldehyde to partially hydrolysed nylon 6/6 tubes. The highest activity of immobilized enzyme was obtained at 65?°C and pH 6.5, while the optimum temperature for free urease was found to be 25?°C. Immobilized urease showed an improved thermal stability in comparison to free urease. It retained 76% of the original activity after 60 days when stored at 4?°C and 78% of the activity after 5 repeated uses. |
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