首页 | 本学科首页   官方微博 | 高级检索  
     


Destruction of photosystem I iron-sulfur centers of spinach andAnacystis nidulans by mercurials
Authors:Yasuo Kojima  Yuri Niinomi  Shigeru Tsuboi  Tetsuo Hiyama  Hidehiro Sakurai
Affiliation:(1) Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, 160 Tokyo;(2) Department of Biochemistry, Faculty of Science, Saitama University, Urawa, 338 Saitama;(3) Present address: Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153 Tokyo
Abstract:Several mercurials destroyed Photosystem I (PSI) Fe−S centers in thylakoids and PSI particles from spinach and fromAnacystis nidulans as revealed by EPR measurement and acid-labile sulfide determination. Of the mercurials tested, HgCl2 was the most effective, followed by phenylmercuric acetate (PMA), Mersalyl and pCMB in the order of decreasing effectiveness. Fe−S centers in thylakoids were much more labile than those in PSI particles. InA. nidulans thylakoids, Center B was more susceptible than Center A and X to PMA. P700 was less susceptible to PMA than these centers. For 50% inactivation of Fe−S centers inA. nidulans thylakoids, about 0.4 mM PMA was required for Center B, and about 1 mM was required for Center A and X. These differential susceptibilities of Fe−S centers were more pronounced with HgCl2 than with the other three mercurials.
Keywords:Anacystic nidulans   Inhibitors of photosynthesis  Iron-sulfur centers  Mercurials  Photosystem I  Spinach chloroplasts
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号