Destruction of photosystem I iron-sulfur centers of spinach andAnacystis nidulans by mercurials |
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| Authors: | Yasuo Kojima Yuri Niinomi Shigeru Tsuboi Tetsuo Hiyama Hidehiro Sakurai |
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| Affiliation: | (1) Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, 160 Tokyo;(2) Department of Biochemistry, Faculty of Science, Saitama University, Urawa, 338 Saitama;(3) Present address: Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153 Tokyo |
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| Abstract: | Several mercurials destroyed Photosystem I (PSI) Fe−S centers in thylakoids and PSI particles from spinach and fromAnacystis nidulans as revealed by EPR measurement and acid-labile sulfide determination. Of the mercurials tested, HgCl2 was the most effective, followed by phenylmercuric acetate (PMA), Mersalyl and pCMB in the order of decreasing effectiveness.
Fe−S centers in thylakoids were much more labile than those in PSI particles. InA. nidulans thylakoids, Center B was more susceptible than Center A and X to PMA. P700 was less susceptible to PMA than these centers.
For 50% inactivation of Fe−S centers inA. nidulans thylakoids, about 0.4 mM PMA was required for Center B, and about 1 mM was required for Center A and X. These differential
susceptibilities of Fe−S centers were more pronounced with HgCl2 than with the other three mercurials. |
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| Keywords: | Anacystic nidulans
Inhibitors of photosynthesis Iron-sulfur centers Mercurials Photosystem I Spinach chloroplasts |
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