| 抗轮状病毒鸡卵黄免疫球蛋白耐酸碱性的研究 |
| |
| 引用本文： | 陈红霞,朱斌,慕彩梅.抗轮状病毒鸡卵黄免疫球蛋白耐酸碱性的研究[J].微生物学免疫学进展,2010,38(3):19-20. |
| |
| 作者姓名： | 陈红霞 朱斌 慕彩梅 |
| |
| 作者单位： | 1. 深圳新鹏生物工程有限公司,深圳,518057
2. 深圳市安拓兴生物技术开发有限公司,深圳,518020 |
| |
| 摘 要： | 在pH2～12不同酸碱度环境中37℃保温0.5、1.0、2.0、3.0、4.0h后对抗轮状病毒鸡卵黄免疫球蛋白(IgY)的中和效价、分子结构变化及其耐酸耐碱性进行了测定。结果显示,在pH2～9范围内,37℃保温至4h,抗轮状病毒IgY的中和效价无明显变化,SDS-PAGE非还原性电泳图中未出现降解带;pH≥10时,37℃保温至1h,中和抗体效价即开始下降,电泳图中出现降解带,pH达11、12时处理1h以上样品则全部降解,生物活性丢失。提示抗轮状病毒IgY的耐酸性较耐碱性强,能耐受正常人体消化道内酸碱度。为制备成口服制剂提供试验依据。
|
| 关 键 词： | 轮状病毒 鸡卵黄抗体(IgY) 耐酸碱性 |
The behavior of anti-human rotavirus IgY resisting acid and alkali |
| |
| CHEN Hong-xia,ZHU Bin,MU Cai-mei.The behavior of anti-human rotavirus IgY resisting acid and alkali[J].Progress In Microbiology and Immunology,2010,38(3):19-20. |
| |
| Authors: | CHEN Hong-xia ZHU Bin MU Cai-mei |
| |
| Institution: | 1 Shenzhen Xinpeng Bio-tech Co.,Ltd,Shenzhen 518057,China;2 Shenzhen Antitoxin Bio-tech R＆D Co.,Ltd,Shenzhen 518020,China) |
| |
| Abstract: | To investigate the behavior of anti-human rotavirus IgY resisting acid and alkali,and provide the reference for oral IgY.The neutralization titer,molecule structure changes and its acid-alkali resistance were detected after placing IgY in the cases of pH2-12 at 37℃ for 0.5 to 4.0 hours.The results showed that the neutralization antibody titer of anti-rotavirus IgY have no obviously changes,and the degradation band has not been seen in the SDS-PAGE profile under the cases of pH2-9 at 37℃ for 4 hours;the neutralization antibody titer is on the decrease and the degradation band can be seen in SDS-PAGE profile.When IgY was treated in the case of high pH（≥10）for one hour,the samples can be degraded and the bioactivity is lost,this suggests that the acid resistance of IgY is better than its alkali resistance. |
| |
| Keywords: | Rotavirus Chicken egg yolk immunoglobulins（IgY） Behavior resisting acid and alkali |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
