Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation |
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| Institution: | 1. Division of Animal Reproduction, Gynaecology & Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, SKUAST-K, Shalimar, Srinagar, Jammu & Kashmir, 190 006, India;2. Frozen Semen Project, Ranbirbagh Ganderbal, LDBK, Department of Animal Husbandry, Kashmir, 191131, India;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Alej, Svobody 1655/76, 323 00 Pilsen, Czechia;1. Department of Genetics, Faculty of Veterinary Medicine, University of Cordoba, Cordoba 14014, Spain;2. Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba 14014, Spain;3. Instituto de Investigación y Formación Agraria y Pesquera (IFAPA) Hinojosa del Duque, Carretera el Viso, km 2, Córdoba 14270, Spain;1. INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco;2. Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco;3. INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France;4. Departments of Chemistry, Faculty of Science, Mu?la S?tk?Koçman University, 48121 Mu?la, Turkey;1. Laboratorio de Teriogenología “Dr. Héctor H. Morello”, Instituto de Biotecnología Agropecuaria del Comahue (IBAC). Facultad de Ciencias Agrarias, Universidad Nacional del Comahue, Cinco Saltos, Río Negro, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cinco Saltos, Río Negro, Argentina;3. Ministerio de Desarrollo Territorial, Gobierno de la Provincia del Neuquén, Neuquén, Argentina |
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| Abstract: | Ram sperm are subjected to extreme oxidative stress during their preservation at ?196 °C resulting in reduced quality at post thaw. Therefore, the main objective of this study was to evaluate the effect of antioxidants taurine, quercetin and reduced glutathione on the post thaw quality of crossbred ram sperm. A total of twenty four ejaculates from six crossbred rams were collected and extended with tris-based extender with no antioxidant (Control), with taurine (40 mM), quercetin (5 μg/ml) and reduced glutathione (5 mM). The post thaw sperm quality was determined by percent sperm motility, live sperm count, intact acrosome and hypo-osmotic swelling test (HOST) reacted spermatozoa and lipid peroxidation was measured in terms of malondialdehyde (MDA) level both in seminal plasma and sperm cell. At post thaw, percent sperm motility and live sperm count were significantly (p < 0.05) higher for taurine than control and reduced glutathione but did not differ significantly (p > 0.05) from quercetin. The percent HOST reacted spermatozoa were significantly higher for taurine than control, quercetin and reduced glutathione. Seminal plasma MDA level was significantly (p < 0.05) lower for taurine than control and non-significantly lower than quercetin and reduced glutathione. However, spermatic MDA level did not differ significantly (p > 0.05) among the control and antioxidants. In conclusion, taurine at 40 mM reduced lipid peroxidation and improved post thaw sperm quality of cryopreserved crossbred ram semen. Further, transportation time of semen samples in an ice chest at 4–5 °C may be included as a part of equilibration period, when collection shed and frozen semen unit are located at a distance. |
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| Keywords: | Ram Sperm Cryopreservation Antioxidants Oxidative stress Malondialdehyde |
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