Cells isolated from cryopreserved dental follicle display similar characteristics to cryopreserved dental follicle cells |
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| Institution: | 1. Department of Dental Research, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan 650500, PR China;2. National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, PR China;3. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, College of Stomatology, Chongqing Medical University, Chongqing, 401147, PR China;4. Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China;5. Department of Orthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan 650031, PR China;1. Saint Petersburg Institute for Machine Sciences, The Russian Academy of Sciences, Saint Petersburg 199178, Russia;2. Immanuel Kant Baltic Federal University, Kaliningrad 236040, Str. Universitetskaya 2, Russia;1. Postgraduate student, Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;2. Associate Professor, Department of Pedodontics, West China College of Stomatology, Sichuan University; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;3. Postgraduate student, College of Architecture and Environment, Sichuan University; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;4. Postgraduate student, Department of Implantology, West China College of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;5. Postgraduate student, Department of Implantology, West China College of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;6. Postgraduate student, Department of Endodontics, West China College of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;7. Postgraduate student, Department of Endodontics, West China College of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;8. Professor and Director, Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;1. Drug Discovery Informatics Lab, QRC-Qasemi Research Center, Al-Qasemi Academic College, Baka El-Garbiah 30100, Israel;2. Oral and Maxillofacial Surgery Department; Chief of Oral and Maxillofacial Institute, Galilee Medical Center, Nahariya, Israel. Faculty of Medicine in the Galilee, Bar-ilan University, Henrietta Szold 8, P.O.B 1589, 1311502 |
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| Abstract: | Dental follicle tissue is a promising resource of mesenchymal stem cells for cytotherapeutic approaches and tissue engineering applications. There are two procedures for banking of human dental follicle stem cells have been reported. Conventional method requires cell isolation, expansion and immediate cryopreservation. Whereas dental follicle stem cells can be isolated from cryopreserved dental follicle fragments. The aim of this study was to compare the characteristics of dental follicle cells isolated from cryopreserved fragments (DFCs-CF) with dental follicle cells recovered from cryopreserved cells (DFCs-CC). Dental follicle fragments obtained after mechanical disaggregation were divided into two parts, with one part maintained in culture, while another part underwent cryopreservation. Dental follicle fragments and dental follicle cells from fresh tissue were stored in liquid nitrogen for 3 months. After thawing, the isolation, morphology, proliferation, cell cycle, colony-forming-unit ability, stemness-related marker expression, apoptosis, and multi-lineage differentiation potential of DFCs-CF were tested compared with DFCs-CC. DFCs-CF expressed mesenchymal stem cells marker, proliferated well, showed similar levels of mRNA for stemness- and apoptosis-related genes and exhibited the capacity of multi-lineage differentiation similar to those of DFCs-CC. These results imply that cryopreservation of dental follicle fragments is an effective banking method for isolation of dental follicle cells. |
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| Keywords: | Dental follicles Cryopreservation Mesenchymal stem cells |
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