Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos |
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| Institution: | 1. Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Camino Cruz Del Sur, 2250, Montevideo, Uruguay;2. Programa de Posgrado, Facultad de Veterinaria, Universidad de La República, Av. Lasplaces, 1550, Montevideo, Uruguay;3. Unidad de Animales Transgénicos y de Experimentación, Institut Pasteur de Montevideo, Mataojo, 2020, Montevideo, Uruguay;1. Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju, Gyeongnam Province 660-701, Republic of Korea;2. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam Province 660-701, Republic of Korea;3. Animal, Dairy, and Veterinary Sciences Department, Utah State University, Logan, UT 84322-4700, USA;4. School of Veterinary Medicine, Utah State University, Logan, UT 84322-4700, USA;5. Center for Integrated Biosystems, Utah State University, Logan, UT 84322-4700, USA;1. Unidade de Biotecnologia e Recursos Genéticos, INIAV-Santarém, Quinta da Fonte Boa, Vale de Santarém, Portugal;2. Escola de Ciências e Tecnologia, ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Núcleo da Mitra, Évora, Portugal;3. CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa Avenida da Universidade Técnica, Lisboa, Portugal;4. Escola Universitária Vasco da Gama, Av. José R. Sousa Fernandes, Campus Universitário – Bloco B, Lordemão, Coimbra, Portugal;1. Embrapa Goats and Sheep, Núcleo Regional Sudeste, CEJHB–Embrapa Gado de Leite, Coronel Pacheco, MG, Brazil;2. Faculty of Veterinary Medicine, Fluminense Federal University, Niterói, RJ, Brazil;3. Department of Preventative Veterinary Medicine and Animal Reproduction, College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, SP, Brazil;4. Department of Preventive Veterinary Medicine, College of Veterinary - Minas Gerais Federal University, Belo Horizonte, MG, Brazil;5. Department of Animal and Wildlife Sciences, University of Pretoria, Pretoria, South Africa;1. Texas Tech University, 2500 Broadway, Lubbock, TX 79409, United States;2. Texas Tech University Health Sciences Center, 3601 4th St, Lubbock, TX 79430, United States;1. School of Health, Medical & Applied Sciences, Central Queensland University, Rockhampton, QLD 4702, Australia;2. National Key Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Hanoi, Viet Nam;3. Australian Reproductive Technologies, Mt Chalmers, QLD 4702, Australia;4. Education Program in Reproduction & Development, Department of Obstetrics & Gynaecology, Monash University, Clayton, VIC 3168, Australia |
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| Abstract: | The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 in vivo and 240 in vitro produced embryos were cryopreserved either by conventional freezing, or by vitrification with Cryotop or Spatula MVD methods on Day 6 after insemination/fertilization. After thawing/warming and transfer, embryo survival rate on Day 30 of gestation was affected by the source of the embryos (in vivo 53.3%, in vitro 20.8%; P < 0.05) and by the method of cryopreservation (conventional freezing 26.5%, Cryotop 52.0%, Spatula MVD 22.2%; P < 0.05). For in vivo derived embryos, survival rate after embryo transfer was 45.6% for conventional freezing, 67.1% for Cryotop, and 40.4% for Spatula MVD. For in vitro produced embryos, survival rate was 7.3% for conventional freezing, 38.7% for Cryotop, and 11.4% for Spatula MVD. Fetal loss from Day 30 to birth showed a tendency to be greater for in vitro (15.0%) rather than for in vivo produced embryos (5.7%), and was not affected by the cryopreservation method. Gestation length, weight at birth and lamb survival rate after birth were not affected by the source of the embryo, the cryopreservation method or stage of development (average: 150.5 ± 1.8 days; 4232.8 ± 102.8 g; 85.4%; respectively). This study demonstrates that embryo survival and birth rate of both in vivo and in vitro produced ovine embryos are improved by vitrification with the minimum volume Cryotop method. |
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| Keywords: | Sheep Lambs IVP Cryotolerance Ethylene glycol DMSO |
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