Validated spectrofluorimetric and resonance Rayleigh scattering methods for determining naftidrofuryl in varied pharmaceutical samples based on its interaction with erythrosin B |
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Authors: | Mona Saad Binkadem Huda Salem AlSalem Soha Talal Al-Goul Mohamed A El Hamd Mohamed Oraby Faten M Ali Zainy Mohamed A Abdel-Lateef |
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Institution: | 1. Department of Chemistry, College of Science, University of Jeddah, P.O. Box 80327, Jeddah, Saudi Arabia;2. Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, Saudi Arabia;3. Department of Chemistry, College of Sciences & Arts, King Abdulaziz University, Rabigh, Saudi Arabia;4. Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Shaqra, 11961 Saudi Arabia;5. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524 Egypt;6. Chemistry Department, Faculty of Science, University of Jeddah, P.O. Box 80327, Jeddah, 21589 Saudi Arabia;7. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, 71524 Egypt |
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Abstract: | Naftidrofuryl is a vasodilator medication used for treating cerebral and peripheral vascular diseases. In this study, two spectroscopical techniques, spectrofluorimetric and resonance Rayleigh scattering (RRS), were utilized to quantify naftidrofuryl in its pharmaceutical samples. The developed methodologies in this study rely on a facile process of forming an association complex between erythrosine B reagent and naftidrofuryl under acidic conditions. The fluorimetric assay is based on the ability of naftidrofuryl to quench and decrease the native fluorescence intensity of the reagent when measured at = 550 nm ( = 526 nm). Under similar reaction conditions, the RRS method relies on the observed amplification in the RRS spectrum of the reagent at a wavelength of 577 nm following its interaction with naftidrofuryl. The methods exhibited linearity within the ranges 0.2–1.6 μg/ml (r2 = 0.999) and 0.1–1.4 μg/ml (r2 = 0.9994), with limit of quantitation values of 0.146 and 0.099 μg/ml, and limit of detection values of 0.048 and 0.032 μg/ml, for the fluorometric and the RRS methods, respectively. Moreover, the quenching between the dye and naftidrofuryl was studied using Stern–Volmer analysis, and the methodologies were experimentally optimized and validated. Additionally, acceptable recoveries were achieved when the procedures were applied to determine naftidrofuryl in pharmaceutical samples. |
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Keywords: | erythrosine B naftidrofuryl pharmaceutical samples resonance Rayleigh scattering spectrofluorimetry |
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