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Differential expression of the Sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants |
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| Authors: | Krzysztof Szczyglowski Trevor Potter Jon Stoltzfus Susan Y Fujimoto Frans J de Bruijn |
| Institution: | (1) Department of Energy Plant Research Laboratory, Michigan State University, 48824-1312 East Lansing, MI, USA;(2) Department of Microbiology, Michigan State University, 48824-1312 East Lansing, MI, USA;(3) Genetics Program, Michigan State University, 48824-1312 East Lansing, MI, USA |
| Abstract: | The involvement of the Sesbania rostrata glb3 gene promoter NICE (nodule-infected cell expression) element in root-enhanced expression of 5 -Srglb3-uidA-3nos chimeric gene was investigated in transgenic Nicotiana tabacum plants. The full-length wild-type Srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. The expression pattern of nine selected Srglb3 promoter mutations in the NICE element was examined in transgenic tobacco plants and compared with the pattern observed in nodules of transgenic Lotus corniculatus plants. The results suggest that the highly conserved motifs in the NICE element play an important role in expression in roots of non-legume plants. |
| Keywords: | leghemoglobin gene expression site-directed mutagenesis |
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