Differential expression of keratin genes during mouse development |
| |
| Authors: | T Ouellet M Lussier C Bélanger A Kessous A Royal |
| |
| Affiliation: | 1. Department of Dermatology, University of Tartu, 31 Raja Street, 50417 Tartu, Estonia;2. Dermatology Clinic, Tartu University Hospital, 31 Raja Street, 50417 Tartu, Estonia;3. Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia;4. Department of Reproductive Biology, Estonian University of Life Sciences, 62 F. R. Kreutzwaldi Street, 51014 Tartu, Estonia;1. Department of Dermatology, University of Tartu, 31 Raja Street, 50417 Tartu, Estonia;2. Dermatology Clinic, Tartu University Hospital, 31 Raja Street, 50417 Tartu, Estonia;5. Department of Pathology, Tartu University Hospital, 8 L. Puusepa Street, 51014 Tartu, Estonia;1. Department of Dermatology, University of Tartu, 31 Raja Street, 50417 Tartu, Estonia;2. Dermatology Clinic, Tartu University Hospital, 31 Raja Street, 50417 Tartu, Estonia;1. Division of Implant Prosthetic Rehabilitation, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;2. Department of Histology, University of Medicine and Pharmacy Craiova, Romania;3. Division of Anatomy, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;4. Department of Prosthodontics, Faculty of Dental Medicine, University of Medicine and Pharmacy Craiova, Romania;1. Departments of Medicine and Biological Chemistry, University of California Irvine, 839 Health Sciences Drive, Irvine, CA 92697-4030, USA;1. Department of Plastic and Aesthetic Surgery, The University of Tokyo Hospital, Japan;2. Gene expression Laboratory, Salk Institute for Biological Studies, United States;3. Institute for Advanced Co-Creation Studies, Osaka University, Japan;4. Graduate School of Engineering Science, Osaka University, Japan;3. School of Dentistry, University of California at Los Angeles, Los Angeles, California 90095;5. Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, California 90095;6. David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 and;4. Kyung Hee University, School of Dentistry, Seoul, 130-701, Korea;1. Department of Dermatology, University of California San Diego, San Diego, California, USA |
| |
| Abstract: | Suprabasal layers of the newborn mouse epidermis contain two mRNAs of 2.0 and 2.4 kb which are translated into keratins of 59 and 67 kDa, respectively. To study their expression during development, cDNA sequences corresponding to the 2.0- and the 2.4-kb mRNAs were cloned, characterized by hybridization selection assay, and used as probes to detect keratin sequences in polyadenylated RNA from Day 11, 13, 15, and 17 embryos. In RNA from Day 11 of gestation, two RNAs of 2.8 and 1.8 kb were identified. They were found to have homologies with both epidermal RNAs, suggesting that they are coding for proteins of the keratin family. These two sequences were not detected in sample of later stages. RNAs comigrating with the two epidermal keratin RNAs were identified only in Day 15 and 17 embryos indicating that their expression was induced between Day 13 and 15. Finally, the localization of the 59-kDa keratin mRNA was examined by in situ hybridization. The spinous and granulous cell layers were found to be heavily covered with grains while other regions of the tissue sections were unlabeled. All these results support the hypothesis of a sequential expression of keratins during differentiation of epidermal cells and suggest that proteins related to the keratins expressed specifically in keratinizing cells are expressed earlier during development. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
