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RasG protein accumulation occurs just prior to amoebae emergence during spore germination in Dictyostelium discoideum |
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| Authors: | Meenal Khosla George B Spiegelman Gerald Weeks Todd W Sands Kiran J Virdy David A Cotter |
| Institution: | Department of Microbiology, University of British Columbia, Vancouver, B.C. V6T 1Z3, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, B.C. V6T 1Z3, Canada; Department of Biological Sciences, University of Windsor, Windsor, Ontario N9B 3P4, Canada |
| Abstract: | Abstract RasG protein levels in dormant and germinating spores of Dictyostelium discoideum strains JC1 and SG1 were estimated by Western blotting. Ras Glevels were very low in dormant spores and remained low during the lag period, regardless of whether spores were heat activated or treated with autoactivator during the early stages of spore germination. RasG levels increased late during spore swelling just prior to the emergence stage of germination. These data are consistent with a requirement for RasG during vegetative growth. |
| Keywords: | Dictyostelium discoideum RasG protein Western blotting Germination |