细胞核受体LXRβ的体外酶标测活方法的建立与应用

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Organophosphorus Pesticides (Ops) Operate to Arabidopsis Thaliana by the Different Expression Protein and Inhibiting the Activity of Esterase Isoenzymes

Plants can tolerate or even degrade organophosphorus (OPs), yet with little known about the mechanisms. Employing Arabidopsis thaliana, a model plant, this paper studied the response of the esterase isozymes and the total soluble proteins to OPs stress. The results showed that OPs could be uptooke by Arabidopsis thaliana roots rapidly into the plant, repressed the activity of esterase isoenzyme without reduction in the enzyme content, promoted the production of one different protien highly homologous to the large-subunit sequence of its own ribulose-1,5-bisphophate carboxylase/oxygenase (RuBisCO) and finally, markedly inhibited the plant development. Also, the negative effects of OPs on the plant enhanced with the increase of OPs amount and treating time. This strongly suggested that esterase isoenzyme. probably was the target enzyme of OPs, like in animal, and the different expression protein may also be involved in the response to OPs.