Instability of actinomadurae protoplasts generated by lytic enzyme treatment |
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| Authors: | DG Allison PM Roberts |
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| Institution: | Pharmaceutical Sciences Institute, Aston University, Birmingham B4 7ET;Pharmaceuticals Division, ICI plc, Alderley Park, Macclesfield, SK10 4TG, UK |
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| Abstract: | The effect of lytic enzyme treatment upon protoplast formation and reversion in three species of Actinomadura has been determined. Incubation in the presence of lytic enzyme L2 generates large protoplasts (4 μm diameter) which remain intact for only 2h. In comparison, protoplasts formed by the degradative effects of the enzymes lysozyme and L1 are smaller (2 μm diameter), and remain stable for up to 18h. This results in a greater efficiency of regeneration. Lytic enzyme L2 has been shown to contain impurities, including proteolytic activity, which may affect cell wall regeneration. |
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