长波紫外线照射对花背蟾蜍肾脏结构损伤

为研究长波紫外线对花背蟾蜍(Buforaddei)肾脏的损伤,用波长为365nm的长波紫外线(UVA)以352μW.cm-2的辐射量对花背蟾蜍亚成体进行150、300和450min的连续照射,分别在照射后立即、3、6、9、12和15d取材,常规石蜡切片。结果表明:UVA照射后,3组不同照射时间的花背蟾蜍肾脏整体结构基本完整,但均出现肾小管管壁破裂,管径显著缩小,肾小囊消失,而300min照射组的损伤则极为明显,其后依次为450min照射组与150min照射组,300与450min照射组的肾小体塌陷程度较150min照射组更为严重;经15d的恢复,虽出现明显好转,但大多指标仍与对照组间存在极显著性差异;UVA对花背蟾蜍肾脏有着不可忽视的损伤,虽然具有一定的自我修复的能力,但在野外,紫外辐射的增强和植被的减少可导致两栖类遭受过度的紫外辐射,进而引起两栖类种群的衰减。

Impacts of long-wave ultraviolet radiation on kidney structure of Bufo Raddei

In order to study the damage of long-wave ultraviolet(UVA) on Bufo raddei kidney,three groups of Bufo raddeis juveniles were irradiated continuously by 365 nm UVA with a dosage of 352 μW·cm-2 for 150,300,and 450 min,respectively,and the kidney after the irradiation was dissected immediately and 3,6,9,12,and 15 days later,with its histological changes observed by general histological method.After the UVA radiation,the kidney structure was basically in integrity,except the fracture and visible constriction of renal tubule and the disappearance of renal capsule,which were most obvious in the 300 min group,followed by in 450 min group and in 150 min group.The damage to Malpigkian bodies was more serious in 300 and 450 min groups than in 150 min group.After 15 days recovery,though the damage was somewhat mitigated,most of the histological indices of the three experimental groups were significantly different from the control group.This study showed that the damage of UVA to B.raddei kidney could not be ignored in spite of the kidney’s self-repair capability.In the field,the enhancement of UV radiation and the reduction of vegetation could cause amphibians suffering excessive UV radiation,and even,amphibian population decline.