Magnetic colorimetric immunoassay for human interleukin-6 based on the oxidase activity of ceria spheres |
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Authors: | Juan Peng Jufang Guan Huiqin Yao Xiaoyong Jin |
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Institution: | 1. School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan, 750021, People''s Republic of China;2. Department of Chemistry, Ningxia Medical University, Yinchuan 750004, People''s Republic of China |
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Abstract: | A novel magnetic colorimetric immunoassay strategy was designed for sensitive detection of human interleukin-6 (IL-6) using ceria spheres as labels. Ceria spheres showed excellent oxidase activity, which can directly catalyze the oxidation of substrate o-phenylenediamine (OPD) to a stable yellow product, 2,3-diaminophenazine (oxOPD). The absorbance of oxOPD was recorded to reflect the level of IL-6. The relatively mild conditions made the immunoassay strategy more robust, reliable, and easy. A linear relationship between absorbance intensity and the logarithm of IL-6 concentrations was obtained in the range of 0.0001–10 ng mL−1 with a detection limit of 0.04 pg mL−1 (S/N = 3). The colorimetric immunoassay exhibited high sensitivity and specificity for the detection of IL-6. This immunoassay has been successfully applied in the detection of IL-6 in serum samples and can be readily extended toward the on-site monitoring of cancer biomarkers in serum samples. |
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Keywords: | Ceria sphere Catalytic property Colorimetric immunoassay Human Interleukin-6 |
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