Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH |
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Authors: | Yufeng Yuan Kehan Huang Mengfang Chang Cuifang Qin Sanjun Zhang Haifeng Pan Yan Chen Jianhua Xu |
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Institution: | 1. State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China;2. Tongji Hospital Affiliated to Tongji University, Shanghai, 200065, China |
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Abstract: | Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1–50. |
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Keywords: | Dual-emission fluorescent silver nanoclusters Dual-emission intensity ratio Biological coenzyme NAD+ NAD+/NADH ratios |
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