Analysis of S-methylmethionine and S-adenosylmethionine in plant tissue by a dansylation, dual-isotope method |
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| Authors: | P K Macnicol |
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| Affiliation: | 1. Department of endocrinology, Nanjing Drum Tower Hospital, Chinese Academy of Medical Science & Graduate School of Peking Union Medical College, Nanjing, China;2. Department of endocrinology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China;3. Department of endocrinology. Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China;1. Sri SaiRam Engineering College, Chennai, India;2. Adhiparasakthi Engineering College, Chennai, India;3. MVSR Engineering College, Hyderabad, India;1. Department of Operative Dentistry and Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, China;2. State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China;3. Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada;4. School of Stomatology, Wuhan University, Wuhan, China;1. Environmental Research Center, ICS MAUGERI SPA SB, Institute of Pavia, IRCCS, Pavia, Italy;2. Poison Control Center and National Toxicology Information Center, IRCCS Maugeri Foundation Hospital, Pavia, Italy;3. Department of Forensic Medicine, Pharmacology and Toxicology, University of Pavia, Pavia, Italy;4. Environmental Reasearch Center, ICS Maugeri SPA SB, Institute of Padova, Italy |
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| Abstract: | ![]()
A method is presented for determining the levels of S-methylmethionine (MeMet) and S-adenosylmethionine (AdoMet) in the same plant tissue sample, utilizing readily available equipment. The bottom limit of sensitivity, ca. 100 pmol, can be lowered if required. A trichloracetic acid homogenate of the tissue is supplemented with [carboxyl-14C]MeMet and [carboxyl-14C]AdoMet. After separation of MeMet and AdoMet from each other and from endogenous homoserine on a phosphocellulose column, the two fractions are heat treated at appropriate pH values to liberate [14C]homoserine. Quantitation is via the 3H/14C ratio of [3H]dansyl-[14C]homoserine isolated by thin-layer chromatography. The method is validated with pea cotyledon, corn root, and cauliflower leaf. |
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