| 重组蛋白复性技术研究进展 |
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| 引用本文： | 于德强,何询. 重组蛋白复性技术研究进展[J]. 生物技术通讯, 2004, 15(1): 67-69 |
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| 作者姓名： | 于德强 何询 |
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| 作者单位： | 深圳科兴生物工程股份有限公司,深圳,518057 |
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| 摘 要： | 本对近年来重组蛋白复性技术的研究进行了评述。比较分析了液相和固相复性的各种方法，提出了复性优化的方案，介绍了在化学复性基础上发展物理复性如高压复性法的新思路。
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| 关 键 词： | 表达 重组蛋白 包涵体 液相复性 固相复性 高压复性 |
| 文章编号： | 1009-0002(2004)01-0067-03 |
| 修稿时间： | 2003-07-27 |
The research progress of refolding technologies for recombinant proteins |
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| YU De-qiang,HE Xun Shenzhen Kexing Biotech Co.,Ltd,Shenzhen,China. The research progress of refolding technologies for recombinant proteins[J]. Letters in Biotechnology, 2004, 15(1): 67-69 |
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| Authors: | YU De-qiang HE Xun Shenzhen Kexing Biotech Co. Ltd Shenzhen China |
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| Affiliation: | YU De-qiang,HE Xun Shenzhen Kexing Biotech Co.,Ltd,Shenzhen518057,China |
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| Abstract: | The recent research progress of recombinant protein refolding was reviewed.This paper analyzed various kinds of solution-phase and solid-phase refolding technologies,proposed the refolding optimization procedure and introduced the new solution of physical refolding such as high pressure refolding based upon the chemical refolding. |
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| Keywords: | recombinant protein inclusion body solution-phase refolding solid-phase refolding high pressure refolding |
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