Characterization of sources of resistance to the watermelon strain of Papaya ringspot virus in cucumber: allelism and co-segregation with other potyvirus resistances |
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Authors: | R Grumet E Kabelka S McQueen T Wai R Humphrey |
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Institution: | (1) Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517–353–5568, US |
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Abstract: | At least three sources of resistance to the watermelon strain of Papaya ringspot virus (PRSV-W) have been identified in cucumber (Cucumis sativus L.) including: ’TMG-1’, an inbred line derived from the Taiwanese cultivar, ’Taichung Mou Gua’; ’Dina-1’, an inbred line
derived from the Dutch hybrid ’Dina’; and the South American cultivar ’Surinam’. In this investigation we sought to determine
the inheritance of resistance to PRSV-W in ’Dina-1’, the allelic relationships among the three sources of PRSV-W resistance,
and the relationship between PRSV-W resistance and known resistances to other cucurbit potyviruses. Like ’Surinam’ and ’TMG-1’,
resistance in ’Dina-1’ is controlled by a single gene. Despite differences in dominance vs recessive performance and patterns
of virus accumulation, all three sources of resistance complemented each other. ’TMG-1’ and ’Dina-1’ also possess co-segregating,
single-gene resistances to Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus and Moroccan watermelon mosaic virus. Sequential inoculations and F3 family analysis indicated that resistance to PRSV-W completely co- segregated with resistance to ZYMV in ’TMG-1’. Although
PRSV-W resistances are at the same locus in both ’TMG-1’ and ’Surinam’, ’Surinam’ is only resistant to PRSV-W, and progeny
of ’TMG-1’×’Surinam’ were resistant to PRSV-W but susceptible to ZYMV. Susceptibility to ZYMV and resistance to PRSV-W in
’Surinam’ was not influenced by co-inoculation or sequential in- oculations of the two viruses. Collectively, the co- segregation
of resistances to PRSV-W, ZYMV, WMV and MWMV in ’TMG-1’ (within 1 cM), allelism of PRSV-W resistances in ’TMG-1’ and ’Surinam’,
and resistance to only PRSV-W in ’Surinam’, suggest that multiple potyvirus resistance in cucumber may be due to different
alleles of a single potyvirus resistance gene with differing viral specificities, or that the multiple resistances are conferred
by a tightly linked cluster of resistance genes, of which ’Surinam’ only possesses one member.
Received: 22 July 1999 / Accepted: 2 December 1999 |
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Keywords: | : Cucumis sativus Resistance genes Virus resistance Gene cluster |
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