Characterization and tissue expression of twelve selenoproteins in yellow catfish Pelteobagrus fulvidraco fed diets varying in oxidized fish oil and selenium levels |
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| Institution: | 1. Hubei Hongshan Laboratory, Fishery College, Huazhong Agriculture University, Wuhan 430070, China;2. Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, China;3. Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;1. Department of Biomedical Engineering, Iskenderun Technical University, Hatay 31200, Turkey;2. Department of Genetics, Inonu University School of Medicine, Malatya 44280, Turkey;3. Department of Molecular Biology and Genetics, Izmir Institute of Technology, Izmir 35430, Turkey;1. Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil;2. National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil;1. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Patología, Av. San Martín 5285, C1417DSM Ciudad Autónoma de Buenos Aires, Argentina;2. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Salud Pública, Av. Chorroarín 280, C1427CWO Ciudad Autónoma de Buenos Aires, Argentina;3. Centro de Estudios Transdisciplinarios de Epidemiología (CETE), Universidad de Buenos Aires, Argentina;1. Department of Physiology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, Nigeria;2. Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria;3. Department of Physiology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria;4. Department of Pharmacology, Faculty of Basic Clinical Sciences, Rivers State University, Port-Harcourt, Nigeria;5. Department of Theriogenology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria;6. Department of Pharmacology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria;7. Department of Physiology, Faculty of Basic Medical Sciences, Chrisland University, Abeokuta, Nigeria;8. Department of Biochemistry, Faculty of Basic Medical Sciences, University of Port-Harcourt, Port-Harcourt, Nigeria;9. Department of Physiology, Faculty of Basic Medical Sciences, Achievers University, Owo, Ondo, Nigeria;1. Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China;2. Department of Emergency, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen, China;3. Department of General Surgery, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai, China;4. Bio-analytical Laboratory, Shantou University Medical College, Shantou, China |
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| Abstract: | BackgroundSelenium (Se) functions through selenoproteins and is essential to growth and metabolism of vertebrates. The present study was conducted to identify twelve selenoproteins genes (selenoe, selenof, selenoh, selneoi, selenom, selenok, selneon, selenoo, selenot, selenos, selenou and msrb1) from yellow catfish. Their mRNA expression patterns, as well as their response to dietary oxidized fish oils and Se addition were explored.MethodsWe use 3′and 5′ RACE PCR to clone full-length cDNA sequence of twelve selenoprotein genes from yellow catfish. Their mRNA expression patterns were assessed via quantitative real-time PCR. Yellow catfish were fed diet adequate Se+ fresh fish oil, adequate Se+ oxidized fish oil, high Se+ fresh fish oil and high Se+ oxidized fish oil, respectively, for 10 weeks. Their kidney, heart, brain and testis were used to assess the mRNA expression of twelve selenoprotein.ResultsTwelve selenoprotein genes had similar domains with mammals and the other fish. Their mRNAs were expressed widely in eleven tissues but varied with the tissues. Dietary oxidized fish oils and Se addition influenced their mRNA abundances of twelve selenoproteins in a tissue-dependent manner.ConclusionOur study demonstrated the characterization and expression of twelve selenoproteins, and elucidated their responses in yellow catfish fed diets varying in oxidized fish oils and Se addition, which increased our knowledge into the biological function and regulatory mechanism of Se and selenoproteins in fish. |
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