Decreased Frequency of Intestinal Regulatory CD5+ B Cells in Colonic Inflammation |
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| Authors: | Yoshiyuki Mishima Shunji Ishihara Akihiko Oka Nobuhiko Fukuba Naoki Oshima Hiroki Sonoyama Noritsugu Yamashita Yasumasa Tada Ryusaku Kusunoki Ichiro Moriyama Takafumi Yuki Kousaku Kawashima Yoshikazu Kinoshita |
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| Institution: | 1. Department of Internal Medicine II, Shimane University Faculty of Medicine, Izumo, Shimane, Japan;2. Cancer Center, Shimane University Hospital, Izumo, Shimane, Japan;Massachusetts General Hospital, UNITED STATES |
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| Abstract: | BackgroundCD5+ B cells are a type of regulatory immune cells, though the involvement of this B cell subset in intestinal inflammation and immune regulation is not fully understood.MethodsWe examined the distribution of CD5+ B cells in various mouse organs. Expression levels of CD11b, IgM, and toll-like receptor (TLR)-4 and -9 in B cells were evaluated. In vitro, TLR-stimulated IL-10 production by colonic lamina propria (LP) CD5+ and CD5- B cells was measured. In vivo, mice with acute or chronic dextran sulfate sodium (DSS)-induced colonic injury were examined, and the frequency of colonic LP CD5+ B cells in those was assessed by flow cytometry.ResultsThe expression level of TLR9 was higher in colonic LP CD5+ B cells as compared to CD5- B cells. Colonic LP CD5+ B cells produced greater amounts of IL-10 following stimulation with TLR ligands, especially TLR9, as compared with the LP CD5- B cells. Acute intestinal inflammation transiently decreased the frequency of colonic LP CD5+ B cells, while chronic inflammation induced a persistent decrease in colonic LP CD5+ B cells and led to a CD5- B cell-dominant condition.ConclusionA persistent altered mucosal B cell population caused by chronic gut inflammation may be involved in the pathogenesis of inflammatory bowel diseases. |
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