One-step synthesis of Gly-Gly-PheNH2 from N-unprotected amino acid derivatives by papain in one-phase liquid media |
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| Authors: | Pedro Lozano José L Iborra Arturo Manjón Didier Combes |
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| Institution: | (1) Departmento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química, Universidad de Murcia, Ap 4021, Murcia, España;(2) Department de Genie Biochimique et Alimentaire, INSA. UA-CNRS 544, Av Rangueil, 31077 Toulouse, France |
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| Abstract: | Summary Papain was able to catalyze the one-step synthesis of Gly-Gly-PheNH2, from N-unprotected amino acid derivatives. Maximum synthetic activity was obtained for a pH value of 6.5 and for a ]PheNH2]/Gly-GlyOEt] ratio of 6. The presence of an organic cosolvent, such as ethylene glycol, influenced the synthetic activity. Synthetic yield was higher than 65% for a 12.5 M cosolvent concentration. |
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