Plant growth regulators and Axl and immune checkpoint inhibitors from the edible mushroom Leucopaxillus giganteus |
| |
| Authors: | Irine Yunhafita Malya Jing Wu Etsuko Harada Masaaki Toda Corina N D’Alessandro-Gabazza Taro Yasuma |
| |
| Institution: | 1. Graduate School of Science and Technology, Shizuoka University , Shizuoka, Japan;2. Research Institute of Green Science and Technology, Shizuoka University , Shizuoka, Japan;3. Department of Forest and Environmental Science, Miyazaki University , Miyazaki, Japan;4. Department of Immunology, Mie University Graduate School of Medicine , Mie, Japan |
| |
| Abstract: | ABSTRACT A novel compound, (R)-4-ethoxy-2-hydroxy-4-oxobutanoic acid (1), and six known compounds (2–7) were isolated from the fruiting bodies of the wild edible mushroom Leucopaxillus giganteus. The planar structure of 1 was determined by the interpretation of spectroscopic data analysis. The absolute configuration of 1 was determined by comparing specific rotation of the synthetic compounds. In the plant regulatory assay, the isolated compounds (1–7) and the chemically prepared compounds (8–10) were evaluated their biological activity against the lettuce (Lactuca sativa) growth. Compounds 1 and 3–10 showed the significant regulatory activity of lettuce growth. 1 showed the strongest inhibition activity among the all the compounds tested. In the lung cancer assay, all the compounds were assessed the mRNA expression of Axl and immune checkpoints (PD-L1, PD-L2) in the human A549 alveolar epithelial cell line by RT-PCR. Compounds 1–10 showed significant inhibition activity against Axl and/or immune checkpoint. |
| |
| Keywords: | Axl inhibitor immune checkpoint inhibitor plant growth regulator structure determination Leucopaxillus giganteus |
|
|
