Destruction of Pseudomonas aeruginosa pre-formed biofilms by cationic polymer micelles bearing silver nanoparticles |
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Authors: | Tsvetelina Paunova-Krasteva Emi Haladjova Petar Petrov Aleksander Forys Barbara Trzebicka Tanya Topouzova-Hristova |
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Institution: | 1. Bulgarian Academy of Sciences, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria;2. Bulgarian Academy of Sciences, Institute of Polymers, Sofia, Bulgaria;3. Polish Academy of Sciences, Centre of Polymer and Carbon Materials, Zabrze, Poland;4. Faculty of Biology, Sofia University St Kliment Ohridski, Sofia, Bulgaria |
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Abstract: | Abstract Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen often associated with biofilm infections. This study evaluated the capacity for biofilm destruction of a novel combination of cationic polymer micelles formed from poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA-PCL-PDMAEMA) triblock copolymer either alone, or loaded with silver nanoparticles (M_AgNPs). Pre-formed P. aeruginosa biofilms were incubated with either blank micelles, AgNO3, or M_AgNPs. Biofilm biomass (crystal violet assay), metabolic activity (Alamar blue reduction), structure (SEM) and viability (CLSM after Live/Dead staining, or plating for CFU) were checked. The results showed that the micelles alone loosened the biofilm matrix, and caused some alterations in the bacterial surface. AgNO3 killed the bacteria in situ leaving dead biofilm bacteria on the surface. M_AgNPs combined the two types of activities causing significant biofilm reduction, and alteration and death of biofilm bacteria. Therefore, the applied PDMAEMA-based micelles appear to be a successful candidate for the treatment of P. aeruginosa biofilm infections. |
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Keywords: | AgNPs-bearing cationic polymer micelles P aeruginosa biofilm destruction PDMAEMA |
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