Immobilization of Rhodococcus equi DSM 89-133 onto porous celite beads for cholesterol side-chain cleavage |
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| Authors: | S Ahmad B N Johri |
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| Institution: | (1) Department of Microbiology, College of Basis Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263 145 UP, India |
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| Abstract: | Summary
Rhodococcus equi cells immobilized onto porous celite beads were active in side-chain cleavage of cholesterol. The effect of bead size, adsorption time and washing cycles were examined. The degradation of cholesterol side-chain using adsorbed cells was studied in batch and semi-continuous systems. Compared to free cells (80 mol%), the end-product (1,4-androstadiene-3,17-dione and 4-androstene-3,17-dione) profile was lower (70 mol%) with the celite-adsorbed system.
Correspondence to: B. N. Johri |
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