| 不同频率电刺激膈神经导致隔肌肌浆网Ca^2＋—ATPase和Ca^2＋释放—摄取动力学改变 |
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| 作者姓名： | Zhang SY Liu G Wang DL Guo XJ Qian GS |
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| 作者单位： | 张睢扬（第三军医大学新桥医院全军呼吸内科研究所,重庆
400037）;刘刚（第三军医大学新桥医院全军呼吸内科研究所,重庆
400037）;王东林（第三军医大学新桥医院全军呼吸内科研究所,重庆
400037）;郭先健（第三军医大学新桥医院全军呼吸内科研究所,重庆
400037）;钱桂生（第三军医大学新桥医院全军呼吸内科研究所,重庆
400037） |
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| 基金项目： | This work was supported
by the National Natural Science Foundation of China (No.39470325). |
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| 摘 要： | 研究不同频率慢性电刺激（CES）后兔膈肌肌浆网（SR）Ca^2 -ATPase活性以及SRC^2 摄取－释放动力学对不同频率CES的活应性变化,建立不同频率CES组,用定磷法测定SR Ca^2 -ATPaes活性,用Fura-2荧光法测定SR Ca^2 摄取－释放动力学,与对照组比较,慢性低频电刺激10Hz和20Hz组的SR Ca^2 －ATPase活性明显降低（P＜0．01）,Ca^2 释放－摄动力学也显著降低（P＜0．01）,慢性高频电刺激50Hz和100Hz组的SRCa^2 -ATPase活性则显著升高（P＜0．01）,Ca^2 释放－摄取动力学亦明显升高（P＜0．01）,实验提示,ECS后不同频率CES导致膈肌SRCa^2 -ATPase,Ca^2 摄取－释放动力学产生不同的适应性变化,对不同功能状态的膈应用不同频谱的慢性电刺激可能具有重要的临床意义。
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| 关 键 词： | 膈肌疾病 Ca^2＋－ATPase 动力学 慢性电刺激 膈神经 肌浆网 |
| 修稿时间： | 2000年9月18日 |
Changes in SR Ca2+-ATPase activity,Ca2+ release and uptake kinetics of diaphragm muscle after different-frequency chronic electrical stimulation of diaphragmatic nerve |
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| Zhang SY,Liu G,Wang DL,Guo XJ,Qian GS.Changes in SR Ca2+-ATPase activity,Ca2+ release and uptake kinetics of diaphragm muscle after different-frequency chronic electrical stimulation of diaphragmatic nerve[J].Acta Physiologica Sinica,2001,53(3):219-223. |
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| Authors: | Zhang S Y Liu G Wang D L Guo X J Qian G S |
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| Institution: | Institute of Respiratory medicine of Xinqiao Hospital, Third Military Medical University, Chongqing 400037. suiyangzhang@yahoo.com |
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| Abstract: | To study adaptation of rabbit diaphragm muscle after different-frequency chronic electrical stimulation, Ca(2+)-ATPase activity and Ca(2+) release-uptake kinetics of sarcoplasmic reticulum (SR) were respectively measured by detecting inorganic phosphorus ion and Furo-2 fluorescence. SR Ca(2+)-ATPase activity of the low-frequency stimulation group was significantly lower than that of the control group (P<0.0l), but it was significantly higher in the high-frequency stimulation group against control (P<0.0l). The kinetics of Ca(2+) release and Ca(2+) uptake was significantly lower in low-frequency group than that of the control (P<0.0l), but the kinetics of Ca(2+) release and Ca(2+) uptake was significantly higher than that of the control (P<0.01). It is thought that different-frequency electrical stimulation induced different adaptative changes in SR Ca(2+)-ATPase activity, and Ca(2+) release and uptake kinetics of rabbit diaphragm muscle. |
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