Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5 |
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| Authors: | Nir Dover Jason R. Barash Karen K. Hill Karen W. Davenport Hazuki Teshima Gary Xie Stephen S. Arnon |
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| Affiliation: | 1Infant Botulism Treatment and Prevention Program, California Department of Public Health, Richmond, California, United States of America;2Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America;Institute Pasteur, France |
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| Abstract: | Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters. |
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