A Biochemical Evidence of the Inhibitory Effect of Diflubenzuron on the Metamorphosis of the Silkworm,Bombyx mori |
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| Institution: | 1. Department of Sericulture and Entomology Resources, Sangju National University, Sangju, Korea;2. School of Bioresource Sciences, Andong National University, Andong, Korea;1. Department of Virology, Biological Faculty, Moscow State University, Moscow, 119992, Russia;2. Molecular Plant Pathology Laboratory, USDA-ARS, Beltsville, MD, 20705, USA;3. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia;4. Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, 119991, Russia;1. Department of Chemistry, R. K. Mission Residential College, Narendrapur, Kolkata 700103, India;2. Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34-36, D-45470 Mülheiman der Ruhr, Germany;1. Department of Chemistry, Faculty of Science, Imam Khomeini International University, Qazvin, Iran;2. Department of Chemistry, Malek-Ashtar University of Technology, Tehran, Iran;1. Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom;2. Micro-/Nano-Electrochemistry – Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, D-44780 Bochum, Germany;1. Université de Strasbourg, CNRS, IBMP UPR 2357, F-67000 Strasbourg, France;2. Università di Bologna, Dipartimento di Scienze e Tecnologie Agroambientali, Viale G. Fanin 40, 40127 Bologna, Italy;1. School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, China;2. State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, China |
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| Abstract: | Diflubenzuron (DFB) has been known to prevent metamorphosis of silkworm, Bombyx mori, from larval to pupal stage at low dose exposure. To explain this inhibitory action of DFB, a hypothesis was raised that DFB acts like juvenile hormone (JH) or DFB inhibits JH esterase to increase endogenous JH titer. A JH bioassay using isolated abdomen clearly indicates that DFB does not act as JH analog because DFB did not induce vitellogenesis in the isolated female abdomen, while endogenous JHs did significantly. General esterase activities in hemolymph were lower in DFB-treated fifth instar larvae than in the control larvae, but there was no difference between fat body esterase activities in both groups. Two hemolymph esterases (‘E1’ and ‘E2’) of the fifth instar larvae were separated and visualized by α-and β-naphthyl acetate. From in vitro incubation experiment, the cathodal esterase (‘E1’) was sensitive to DFB at its nanomolar range. Considering the fact that early fifth instar larvae have high level of JH esterase in the hemolymph, these results suggest that DFB inhibit larval to pupal metamorphosis by blocking JH degradation, which increases endogenous JH titer especially at the critical period when the larvae determine metamorphic development at the following molt. |
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