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Semen cryopreservation and artificial insemination in budgerigars (Melopsittacus undulatus) |
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| Authors: | J. H. SAMOUR J. A. MARKHAM H. D. M. MOORE and P. F. WATSON |
| Affiliation: | MRC/AFRC Comparative Physiology Research Group, The Institute of Zoology, The Zoological Society of London, Regent's Park, London, NWl 4RY;Department of Physiology, Biochemistry and Pharmacology, The Royal Veterinary College, Royal College Street, London, NWl OTU |
| Abstract: | Semen samples were obtained from budgerigars. Undiluted samples were used directly for artificial insemination (AI) while other samples were diluted with a modified glycerolated BWW and Lake's freezing medium and subjected to two different cryopreservation protocols. Micro-dialysis was used post-thawing to remove the glycerol present in the medium. The assessment of glycerol concentration during micro-dialysis was carried out by means of an ultraviolet spectrophotometry method. Functional assessment of spermatozoal fertilizing capacity post-thawing included the measurement of spermatozoal swimming speed and AI. |
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