基因芯片在细菌基因组学和细胞微生物学研究中的应用

利用基因芯片可以鉴定病原菌在不同宿主微环境中受到差异调节的基因、直接或间接由转录因子控制的基因,以及编码多步骤代谢和生物合成途径中各种组分的基因;通过比较基因组学研究,评价相关菌种和菌株的自然群体内遗传多样性的范围和特性,并在ORF水平描述病原体和共生体之间的差异;同时也可进行感染组织细胞基因表达分析,研究病原体和宿主之间复杂的相互作用关系。

Application of microarray in research on bacterial genomics and cellular microbiology

Microarray expression profiling is a powerful method to identify genes differentially regulated in different host microenvironments, genes that are controlled, directly or indirectly, by transcription factors and genes that code for components of multistep metabolic and biosynthetic pathways. Equally illuminating are microarray-based comparative studies to assess the extent and nature of genetic variability within natural populations of related species and strains and to delineate differences, at the ORF level of resolution, between pathogen and commensal. Expression studies of infected host cells have been conducted as well to analyze the complex interaction between pathogenic bacteria and host.