Temperature gradient DNA-probe column chromatography: as tools for detection and purification of particular DNAs and RNAs |
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| Authors: | H Tsurui A Suyama A Wada |
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| Institution: | Department of Physics, Faculty of Science, University of Tokyo, Japan. |
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| Abstract: | We have developed the temperature-gradient DNA-probe column chromatography as the new method for detecting and purifying particular DNAs or RNAs accurately. The method has high discrimination resolution, and can detect a single-base change in sample base sequences. The method also has high performances in purification of particular DNAs or RNAs, which has been demonstrated by purification of bovine mitochondria serine t-RNA. None of other method can succeed in complete purification of this t-RNA molecule. The present method can be applied not only to molecular biology as basic tools for purifying and picking-up particular sequences but also to the medical field as diagnostic tools. |
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