Allelic polymorphism of murine IgE controlled by the seventh immunoglobulin heavy chain allotype locus |
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Authors: | Mario Sanchez Borges Yoshihiro Kumagai Ko Okumura Nakami Hirayama Zoltan Ovary Tomio Tada |
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Institution: | (1) Department of Immunology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan;(2) Department of Pathology, New York University School of Medicine, 10016 New York, New York |
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Abstract: | Two alloantisera against hybridoma-derived IgE detected allotypic determinants expressed on the murine s chain. An antiserum raised in BALB/c mice against monoclonal IgE of C57BL/6 origin reacted exclusively with IgE of strains having Igh-1b (IgG2a) allotype. The second antiserum, C57BL/6 anti-BALB/c monoclonal IgE, reacted with IgE of strains having Igh-1a, Igh-1d, Igh-1e and Igh-1j allotypes. The genetic studies of (BALB/c x C57BL/6)F1 and backcross F2 animals indicated that the locus controlling the IgE allotype is linked to the Igh-1 locus. This was further confirmed by the possession of respective IgE allotypes by Igh-C congenic mice, BALB/c and BAB-14, C3H.SW/Hz and CWB/Hz. Thus, the allotype detected on the chain is controlled by the seventh murine immunoglobulin allotype locus, and should be designated as the Igh-7 allotype.Abbreviations used in this paper PCA
passive cutaneous anaphylaxis
- RID
radioimmunodiffusion
- i.p.
intraperitoneally
- EA
egg albumin
- Igh-C
immunoglobulin heavy chain constant region locus
- DNP
2,4-dinitrophenyl
- PBS
phosphate-buffered saline
- NMS
normal mouse serum
- KLH
keyhole limpet hemocyanin
Visiting investigator supported by the Scientific and Humanistic Development Council from the Central University of Venezuela, currently at the following address: Consejo de Desarrollo Cientifico y Universidad Central de Venezuela, Av. Principal Urb. La Floresta Ota., Silenia Caracas, Venezuela. |
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